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Esterification of benzoic acid and glycerol to α-monobenzoate glycerol in solventless media using an industrial free Candida antarctica lipase B

Paper ID Volume ID Publish Year Pages File Format Full-Text
34927 45060 2012 8 PDF Available
Title
Esterification of benzoic acid and glycerol to α-monobenzoate glycerol in solventless media using an industrial free Candida antarctica lipase B
Abstract

The enzymatic reaction of benzoic acid and glycerol in the absence of organic solvents to obtain the 1- or 3-monobenzoate glycerol (α-MBG) is studied. Esterification runs were batch wise performed with a concentration of enzyme of 30 g/L, changing the initial concentration of the acid in glycerol from 20 to 60 g/L, and temperature from 50 to 70 °C. In these conditions, the most active lipase among those tested for this synthesis was lipase B isozyme from Candida antarctica (CALB), obtaining conversion values higher than 80% and a significant selectivity to α-MBG. Unlike the synthesis in organic media, water did not have an inhibitory behaviour; hence control of water activity was unnecessary. Temperature and benzoic acid act synergically as deactivating factors. Considering the aforementioned, a kinetic model according to a Michaelis–Menten mechanism is proposed. This model considers a partial enzymatic deactivation mechanism with two terms, one of them accounting for the deactivating action of the acid.

► Free CALB industrial lipase is able to synthesize 1-monobenzoate glycerol in solventless medium. ► CALB lipase is deactivated by both temperature and benzoic acid. ► Kinetics are explained by a simple Michaelis–Menten model with a coupled reversible first order deactivation model. ► Stability of CALB differs depending on the activity of interest: hydrolytic or synthetic.

Keywords
α-Monobenzoate glycerol; Lipase; Esterification; Enzyme deactivation; Kinetic model
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Esterification of benzoic acid and glycerol to α-monobenzoate glycerol in solventless media using an industrial free Candida antarctica lipase B
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 47, Issue 2, February 2012, Pages 243–250
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us