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Investigation of structural effects and behaviour of Pseudomonas aeruginosa amidase encapsulated in reversed micelles

Paper ID Volume ID Publish Year Pages File Format Full-Text
34930 45060 2012 9 PDF Available
Title
Investigation of structural effects and behaviour of Pseudomonas aeruginosa amidase encapsulated in reversed micelles
Abstract

The acetohydroxamic acid synthesis reaction was studied using whole cells, cell-free extract and purified amidase from the strains of Pseudomonas aeruginosa L10 and AI3 entrapped in a reverse micelles system composed of cationic surfactant tetradecyltrimethyl ammonium bromide. The specific activity of amidase, yield of synthesis and storage stability were determined for the reversed micellar system as well as for free amidase in conventional buffer medium. The results have revealed that amidase solutions in the reverse micelles system exhibited a substantial increase in specific activity, yield of synthesis and storage stability. In fact, whole cells from P. aeruginosa L10 and AI3 in reverse micellar medium revealed an increase in specific activity of 9.3- and 13.9-fold, respectively, relatively to the buffer medium. Yields of approximately 92% and 66% of acetohydroxamic acid synthesis were obtained for encapsulated cell free extract from P. aeruginosa L10 and AI3, respectively. On the other hand, the half-life values obtained for the amidase solutions encapsulated in reverse micelles were overall higher than that obtained for the free amidase solution in buffer medium. Half-life values obtained for encapsulated purified amidase from P. aeruginosa strain L10 and encapsulated cell-free extract from P. aeruginosa strain AI3 were of 17.0 and 26.0 days, respectively. As far as the different sources biocatalyst are concerned, the data presented in this work has revealed that the best results, in both storage stability and biocatalytic efficiency, were obtained when encapsulated cell-free extract from P. aeruginosa strain AI3 at w0 of 10 were used. Conformational changes occurring upon encapsulation of both strains enzymes in reverse micelles of TTAB in heptane/octanol were additionally identified by FTIR spectroscopy which clarified the biocatalysts performances.

► Whole cells, cell free extract and purified amidase from Pseudomonas aeruginosa strains L10 and AI3 as biocatalysts for acetohydroxamic acid synthesis. ► Specific activity of amidase, product yield and storage stability of amidase solutions were higher when encapsulated in reverse micelles than free in buffer media. ► Enzyme stability and catalytical functions are interrelated and depend on the enzyme structure.

Keywords
Reversed micelles; Pseudomonas aeruginosa amidases; Acetohydroxamic acid; Water content; Stability
First Page Preview
Investigation of structural effects and behaviour of Pseudomonas aeruginosa amidase encapsulated in reversed micelles
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 47, Issue 2, February 2012, Pages 264–272
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering