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Transfer of an adherent Vero cell culture method between two different rocking motion type bioreactors with respect to cell growth and metabolic rates

Paper ID Volume ID Publish Year Pages File Format Full-Text
34933 45060 2012 9 PDF Available
Title
Transfer of an adherent Vero cell culture method between two different rocking motion type bioreactors with respect to cell growth and metabolic rates
Abstract

Rapid and simple cell and virus cultivation can currently be carried out using disposable bioreactors. The CELL-tainer® (CELLution Biotech BV) disposable bioreactor is a rocking-type bioreactor which not only has vertical movement but horizontal displacement as well. Due to this two-directional movement relatively high mass-transfer capacities can be reached when compared with conventional rocking motion-type bioreactors.Using the design of experiments (DoE) approach we have developed models for the mixing times in both the CELL-tainer® and the BIOSTAT® CultiBag RM (Sartorius Stedim Biotech) bioreactor (standard rocking motion-type). The conditions for cultivation of Vero cells in the CELL-tainer® bioreactor were chosen based on comparable mixing times.Vero cells growing adherent to Cytodex 1 microcarriers were cultivated in the CELL-tainer® and in the BIOSTAT® CultiBag RM. Both bioreactors were controlled with regard to temperature, pH and % dissolved oxygen. Vero cell growth in both bioreactors was comparable with respect to the growth characteristics and main metabolite production and consumption rates. Additionally, polio virus production in both bioreactors was shown to be similar.

► Vero cell growth adherent to microcarriers in two different disposable bioreactors. ► Mixing characteristics for both bioreactors were defined using design of experiments. ► Transfer of culture method from Wave-type to CELL-tainer® bioreactor based on mixing. ► Cell growth, metabolic rates and virus production in both systems were comparable. ► Process transfer between rocking motion type bioreactors could be based on mixing.

Keywords
Polio virus; Kinetics; Metabolism; Wave bioreactor; CELL-tainer®
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Transfer of an adherent Vero cell culture method between two different rocking motion type bioreactors with respect to cell growth and metabolic rates
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 47, Issue 2, February 2012, Pages 288–296
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us