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Protective effect of chitosan oligosaccharides against FcɛRI-mediated RBL-2H3 mast cell activation

Paper ID Volume ID Publish Year Pages File Format Full-Text
34938 45060 2012 4 PDF Available
Title
Protective effect of chitosan oligosaccharides against FcɛRI-mediated RBL-2H3 mast cell activation
Abstract

The activation of mast cells by immunoglobulin E-mediated stimuli is considered as a central event in allergic responses. In this regard, chitosan oligosaccharides (COS) of two different molecular weight ranges (1–3 kDa and 3–5 kDa) were investigated for their capabilities against the activation of RBL-2H3 mast cell sensitized with dinitrophenyl-specific immunoglobulin E antibody and stimulated by antigen dinitrophenyl-bovine serum albumin. It was found that COS significantly inhibited RBL-2H3 cell degranulation via attenuating the releases of histamine and β-hexosaminidase. Moreover, the inhibitory activity of COS was accompanied by a reduction in intracellular Ca2+ elevation. Notably, the expression of immunoglobulin Fc epsilon receptor I (FcɛRI) in RBL-2H3 cells was down-regulated by COS treatment in a dose-dependent manner. The suppressive effect of COS on RBL-2H3 cell activation suggested that COS may be potential candidates of novel inhibitors against allergic reactions.

► Mast cell activation plays a pivotal role in allergic responses. ► Chitosan oligosaccharides (COS) inhibit RBL-2H3 mast cell activation. ► Inhibitory effects are due to suppression of RBL-2H3 cell degranulation. ► COS have protective effect against allergic responses.

Keywords
Chitosan oligosaccharides; Histamine; Mast cells; Intracellular Ca2+; FcɛRI
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Protective effect of chitosan oligosaccharides against FcɛRI-mediated RBL-2H3 mast cell activation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 47, Issue 2, February 2012, Pages 327–330
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us