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Comparison of immobilization strategies for Cryptosporidium parvum immunosensors

Paper ID Volume ID Publish Year Pages File Format Full-Text
3494 172 2012 5 PDF Available
Title
Comparison of immobilization strategies for Cryptosporidium parvum immunosensors
Abstract

Cryptosporidium is a waterborne protozoan parasite, which is problematic for the water industry due to widespread environmental presence, low infectious dose and resistance to chlorine disinfection. To replace the existing, slow regulatory monitoring procedure, immunosensors have been proposed. The performance of such sensors is often limited by the antibody immobilization. The aim of this article was to determine the immobilization protocol offering the highest oocyst capture efficiency. Four methods were tested both under static and convective conditions: physisorption, cysteamine–glutaraldehyde linkage, 3-aminopropyl-triethoxysilane functionalization and protein G linkage. The protein G protocol was shown to present the highest recovery rates in both conditions. For the protein G protocol, different antibody concentrations were tested and it was concluded that there is little difference in the recovery rates when the concentration of IgG antibody is higher than 20 μg/mL. Additionally, operation under convective rather than static conditions increased the recovery for every protocol. These results can be useful to inform the design of future biosensor systems, using antibodies as the detection element, for Cryptosporidium.

► First characterization of different protocols for immobilization of Cryptosporidium antibodies. ► Study of both immobilization protocols, antibody concentrations and immobilization conditions. ► Useful to inform the design of future biosensor systems, using antibodies as the detection element, for Cryptosporidium.

Keywords
Immunosensors; Cryptosporidiosis; Protein G; Cryptosporidium parvum; Convective conditions; Static conditions
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Comparison of immobilization strategies for Cryptosporidium parvum immunosensors
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 68, 15 October 2012, Pages 231–235
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us