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Effect of gene knockouts of l-tryptophan uptake system on the production of l-tryptophan in Escherichia coli

Paper ID Volume ID Publish Year Pages File Format Full-Text
34941 45060 2012 5 PDF Available
Title
Effect of gene knockouts of l-tryptophan uptake system on the production of l-tryptophan in Escherichia coli
Abstract

Uptake of l-tryptophan in Escherichia coli was carried out by three distinct permeases, Mtr, TnaB, and AroP, respectively. In the present study, the three genes of l-tryptophan uptake system were knocked out from an l-tryptophan-producing strain of E. coli, respectively. The knockout mutants all showed lower l-tryptophan uptake activities and higher l-tryptophan production than their parent. Among the three genes, the knockout of mtr was most critical for both l-tryptophan uptake and l-tryptophan production. The uptake activity of l-tryptophan of the mtr mutant was 1.5 nmol min−1 (mg dry weight)−1, which was decreased by 48% when compared to that of the parent; the production of l-tryptophan of the mtr mutant was 14.7 g/l, which was increased by 34% when compared to that of the parent. Furthermore, the physiological and fermentation characteristics caused by gene knockouts were also analyzed.

► The knockout mutants of mtr, tnaB, and aroP were, respectively, constructed. ► The mutants all showed lower l-Trp uptake activities than their parent. ► The mutants all showed higher l-Trp production than their parent. ► The knockout of mtr was most critical for both l-Trp uptake and l-Trp production. ► The prevention of l-Trp uptake may be a useful strategy for l-Trp production.

Keywords
l-Tryptophan; Uptake system; Gene knockout; Batch fermentation; Escherichia coli
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Effect of gene knockouts of l-tryptophan uptake system on the production of l-tryptophan in Escherichia coli
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 47, Issue 2, February 2012, Pages 340–344
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us