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In vitro hepatic differentiation of umbilical cord-derived mesenchymal stem cell

Paper ID Volume ID Publish Year Pages File Format Full-Text
34946 45061 2010 8 PDF Available
Title
In vitro hepatic differentiation of umbilical cord-derived mesenchymal stem cell
Abstract

Recently hepatic differentiation of mesenchymal stem cells (MSCs) from bone marrow (BM), adipose tissue (AT), umbilical cord blood (UCB), and umbilical cord (UC) has been reported. In this study, the four-step sequential exposure with oncostatin M (OSM) plus trichostatin A (TSA) or OSM plus dimethyl sulfoxide (DMSO) at the final step induced hepatic differentiation of UC-MSCs. As a result, the morphology and protein expression were sequentially changed in the step-dependent manner. And the urea synthesis rates of (OSM plus TSA)- and (OSM plus DMSO)-treated cells on day 21 reached to 23 ± 0.4 and 20 ± 0.5 μg/106 cells/day, respectively. The ammonia concentrations 24 h after culture with 1 mM NH4Cl-supplemented medium dropped to 0.41 ± 0.08 mM (OSM plus TSA) and 0.57 ± 0.05 mM (OSM plus DMSO). Also the ethoxyresorufin O-deethylase (EROD) activities were 3.4-fold (OSM plus TSA) and 2.2-fold (OSM plus DMSO) higher than non-induced controls on day 21. It is thought that TSA and DMSO cause the expression of key genes through epigenetic change. Although sequential exposure with TSA or DMSO induced some liver-specific functions to some extent, the degree of activities are yet lower than those of mature hepatocytes. So it will be necessary to optimize the concentration and exposure time for achieving comparable activities to normal hepatocytes in the future.

Keywords
Umbilical cord-derived mesenchymal stem cell; Hepatic differentiation; Trichostatin A; Dimethyl sulfoxide; Liver-specific functions
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In vitro hepatic differentiation of umbilical cord-derived mesenchymal stem cell
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 45, Issue 12, December 2010, Pages 1857–1864
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
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Any Questions? feel free to contact us