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Current status of 13C-metabolic flux analysis and future perspectives

Paper ID Volume ID Publish Year Pages File Format Full-Text
34948 45061 2010 9 PDF Available
Title
Current status of 13C-metabolic flux analysis and future perspectives
Abstract

Current 13C-metabolic flux analysis methods were reviewed as well as the weakness of the conventional metabolic flux analysis without 13C-labeled experiments. Although it has been recognized that 13C-labeling technique is powerful in estimating the metabolic fluxes, and the program-based flux analysis is necessary, one may not be confident with the result obtained without experiences and exhaustive trial and errors in practice due to its black box nature. In the present article, we call attention to the importance of investigating the relationships between fluxes and isotopomer or mass isotopomer distributions to understand the mechanism of generating specific isotopomers. Then, the experimental design for the preferred mixture of the specific 13C-labeled substrate was discussed. The effect of the reversibility in the bidirectional flux on the isotopomer distribution was also mentioned, and it was shown why the reliability of the bidirectional fluxes becomes lower. Moreover, by noting that recent development of measurement techniques enables us to measure the isotopomer patterns of intracellular metabolites instead of proteinogenic amino acids, it is mentioned that this enables us to estimate the flux changes during time-variant batch culture. Some future perspectives are discussed in relation to the integration of different levels of information in the cell.

Keywords
Flux analysis; 13C-Labeling experiment; Theoretical flux analysis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 45, Issue 12, December 2010, Pages 1873–1881
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us