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Pyruvate producing biocatalyst with constitutive NAD-independent lactate dehydrogenases

Paper ID Volume ID Publish Year Pages File Format Full-Text
34954 45061 2010 4 PDF Available
Title
Pyruvate producing biocatalyst with constitutive NAD-independent lactate dehydrogenases
Abstract

Production of pyruvate from lactate through biocatalysis is a valuable process for its simple composition of reaction system and convenience of recovery. Biocatalyst with lactate-induced NAD-independent lactate dehydrogenases (iLDHs) can effectively catalyze lactate into pyruvate. To reduce the cost of biocatalyst preparation caused by indispensable lactate addition, the mutants with constitutive iLDH of Pseudomonas sp. XP-M2 were screened. Mutant XP-LM exhibited high iLDHs activities in minimal salt medium with cheap substrate glucose as the carbon source. The biocatalyst (8.2 g dry cell weight l−1) containing 169.8 U l−1l-iLDH was prepared with 20 g 1−1 glucose. The cost-effective biocatalyst prepared from the mutant XP-LM could efficiently catalyze lactate into pyruvate with high yield (0.961 mol mol−1). Based on the different thermostability of d-iLDH and l-iLDH in the biocatalyst, whole cells of the strain might also have the potential in production of pyruvate and d-lactate from racemic lactate.

Keywords
Biocatalyst; NAD-Independent lactate dehydrogenase; Constitutive expression; Pyruvate; Lactate
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Pyruvate producing biocatalyst with constitutive NAD-independent lactate dehydrogenases
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 45, Issue 12, December 2010, Pages 1912–1915
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us