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Synthesis and application of two light-sensitive copolymers forming recyclable aqueous two-phase systems

Paper ID Volume ID Publish Year Pages File Format Full-Text
34957 45061 2010 9 PDF Available
Title
Synthesis and application of two light-sensitive copolymers forming recyclable aqueous two-phase systems
Abstract

Recycling aqueous two-phase systems (ATPS) are hopeful application techniques in bioseparation and biocatalysis engineering areas. In this study, two novel light-sensitive and reversible water-soluble copolymers were synthesized and used in recycling ATPS. Polymer PNBAC was polymerized by using N-isopropylacrylamide (NIPA), n-butylmethacrylate (BMA), acrylic acid (AA) and chlorophyllin sodium copper salt (CHL) as monomers. Copolymer PNDBC was synthesized by NIPA, 2-(dimethylamino) ethylmethacrylate (DMAEMA), BMA and CHL as monomers. The synthesis yields of two copolymers are 66.9% and 77.2%, respectively. It can be calculated from 1H NMR graph that the molar ratios of monomers in PNBAC (NIPA:BMA:AA:CHL) and PNDBC (NIPA:DMAEMA:BMA:CHL) was 82:4:1:10 and 16:4:3:1, respectively. They could be precipitated by laser irradiation in 488 nm with the least light density of 1.70 × 105 W/m2. The precipitate energy is 1.79 kJ and 3.52 kJ/g, respectively. Five batches of average recoveries of two copolymers in the ATPS are 96.6% and 97.4%. BSA and l-phenylalanine were partitioned in the novel PNDBC–PNBAC ATPS, and their partition coefficients were 2.1 and 0.52. By applying this ATPS to partition of penicillin, the best partition coefficient K is 4.25.

Keywords
Recycling aqueous two-phase systems; Light-sensitive copolymer; Chlorophyllin sodium copper; Bioseparation engineering; Recovery; Partition coefficients
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Synthesis and application of two light-sensitive copolymers forming recyclable aqueous two-phase systems
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 45, Issue 12, December 2010, Pages 1928–1936
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us