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A new approach for the immobilization of permeabilized brewer's yeast cells in a modified composite polyvinyl alcohol lens-shaped capsule containing montmorillonite and dimethyldioctadecylammonium bromide for use as a biocatalyst

Paper ID Volume ID Publish Year Pages File Format Full-Text
34963 45064 2010 5 PDF Available
Title
A new approach for the immobilization of permeabilized brewer's yeast cells in a modified composite polyvinyl alcohol lens-shaped capsule containing montmorillonite and dimethyldioctadecylammonium bromide for use as a biocatalyst
Abstract

Permeabilized brewer's yeast cells were immobilized in modified composite polyvinyl alcohol lens-shaped capsules containing montmorillonite (MONT) and dimethyldioctadecylammonium bromide (DDAB). The special properties of the capsules were then investigated. The results showed that the diffusion capability of hydrophobic molecules from bulk solution into the capsules was obviously improved by the introduction of 0.5% MONT and 0.05% DDAB into the 15% PVA matrix. More importantly, when ethyl 4-chloro-3-oxobutanoate (COBE) was reduced with the capsules as the biocatalyst, the yield and enantiomeric excess (ee) of the product reached 88% and 99.1%, respectively, within 8 h, which are significantly higher totals than those mediated by the unmodified PVA capsules under the same conditions. The intracellular alcohol dehydrogenase (ADH) in the capsules retained over 81% of its original activity after 1 month of storage and 88% of its initial activity after 20 cycles of reaction.

Keywords
PVA, polyvinyl alcohol; MONT, montmorillonite; DDAB, dimethyldioctadecylammonium bromide; ADH, alcohol dehydrogenase; ee, enantiomeric excess; COBE, ethyl 4-chloro-3-oxobutanoate; (S)-CHBE, ethyl (S)-4-chloro-3-hydroxybutanoate; (R)-CHBE, ethyl (R)-4-chlo
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A new approach for the immobilization of permeabilized brewer's yeast cells in a modified composite polyvinyl alcohol lens-shaped capsule containing montmorillonite and dimethyldioctadecylammonium bromide for use as a biocatalyst
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 45, Issue 9, September 2010, Pages 1445–1449
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us