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Promotion of Salvia miltiorrhiza hairy root growth and tanshinone production by polysaccharide–protein fractions of plant growth-promoting rhizobacterium Bacillus cereus

Paper ID Volume ID Publish Year Pages File Format Full-Text
34972 45064 2010 6 PDF Available
Title
Promotion of Salvia miltiorrhiza hairy root growth and tanshinone production by polysaccharide–protein fractions of plant growth-promoting rhizobacterium Bacillus cereus
Abstract

This study was to examine the effects of polysaccharides from a plant growth-promoting rhizobacterium (PGPR) Bacillus cereus on the growth and tanshinone production of Salvia miltiorrhiza hairy roots. A polysaccharide fraction designated BPS was isolated from the hot water extract of B. cereus cells by ethanol precipitation. BPS applied to the root culture at 100–400 mg l−1 a few days before the stationary growth phase stimulated the tanshinone accumulation of roots by about 7-fold (1.59 mg g−1 versus 0.19 mg g−1) and also notably promoted the root growth (15% increase in biomass). BPS was a polysaccharide–protein complex containing about 27% protein, which is essential for root growth promotion. BPS was separated by ultrafiltration into two molecular weight (MW) fractions, of which the high MW fraction (∼35.8 kDa) with higher protein content (∼31%) promoted the root growth while the lower MW fraction with lower protein content (∼17%) suppressed the growth. The results suggest that the polysaccharide portion of BPS was responsible for stimulating the tanshinone accumulation while the protein portion was responsible for promoting the hairy root growth. Polysaccharides from PGPR are potential sources of active elicitors and growth-promoting agents for plant roots in culture.

Keywords
Salvia miltiorrhiza; Hairy root culture; Tanshinones; Bacillus cereus; Polysaccharides; Elicitors
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Promotion of Salvia miltiorrhiza hairy root growth and tanshinone production by polysaccharide–protein fractions of plant growth-promoting rhizobacterium Bacillus cereus
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 45, Issue 9, September 2010, Pages 1517–1522
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us