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Anti-proliferative effect of aminoderivatized chitooligosaccharides on AGS human gastric cancer cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
34973 45064 2010 6 PDF Available
Title
Anti-proliferative effect of aminoderivatized chitooligosaccharides on AGS human gastric cancer cells
Abstract

In this study, the ability of aminoethylation of chitooligosaccharide (COS) to inhibit the proliferation of AGS human gastric adenocarcinoma cells was evaluated. Aminoderivatized COSs, aminoethyl-chitooligosaccharide (AE-COS), dimethyl aminoethyl-chitooligosaccharide (DMAE-COS) and diethyl aminoethyl-chitooligosaccharide (DEAE-COS), were synthesized and confirmed by their IR spectra results in comparison to previous study. Aminoderivatized chitooligosaccharides-induced cell death was characterized by cell viability assay, changes in nuclear morphology and changes in cell morphology. According to our results, all aminoderivatized COSs significantly induced cell death in AGS gastric cancer cells. Moreover, protein and gene expression levels of important regulators involved in apoptosis pathway such as Bcl-2, Bax, p53 and p21 were examined using RT-PCR and Western blot analysis. Aminoderivatized COSs showed dose- and time-dependent inhibition of AGS cancer cell proliferation. AE-COS and DEAE-COS showed the higher apoptotic activity than DMAE-COS. The present results suggest all three kinds of water-soluble aminoderivatized COSs have a promising potential as valuable as cancer chemopreventive agents.

Keywords
Aminoderivatized chitooligosaccharides; Apoptosis; AGS
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Anti-proliferative effect of aminoderivatized chitooligosaccharides on AGS human gastric cancer cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 45, Issue 9, September 2010, Pages 1523–1528
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us