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Identification of nitrifiers and nitrification performance in drinking water biological activated carbon (BAC) filtration

Paper ID Volume ID Publish Year Pages File Format Full-Text
34976 45064 2010 7 PDF Available
Title
Identification of nitrifiers and nitrification performance in drinking water biological activated carbon (BAC) filtration
Abstract

In this study laboratory scale biological activated carbon (BAC) columns were operated with water taken from a surface water reservoir in Istanbul. The aim was to evaluate the efficiency of nitrification in columns packed with two different granular activated carbon grades (open superstructure/chemically activated and closed superstructure/steam activated carbon) and to examine the probable beneficial effect of pre-ozonation. The occurrence and diversity of ammonia-oxidizing bacteria were investigated using 16S rDNA and amoA gene based molecular techniques. Nearly complete removal of NH4+-N was achieved by nitrification in both carbon types. The nitrification efficiency did not change in columns fed with ozonated water. However, the type of feed (either raw or ozonated) played a more important role than the type of GAC with respect to the dominance of nitrifier species in BAC columns. In biofilters ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) were most closely related to Nitrosomonas spp. and Nitrospira spp. as determined by cloning and slot-blot analysis, respectively. The fraction of the AOB population in the biomass was high as detected by real-time PCR. The amoA/16S rDNA ratio varied from 28.7% to 2.1% along the depth of filters. In spite of similar removal efficiencies, BAC columns fed with ozonated water harbored different types of AOB than columns that were receiving raw water.

Keywords
Nitrification; Biological activated carbon; Real-time PCR; Slot-blot hybridization
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Identification of nitrifiers and nitrification performance in drinking water biological activated carbon (BAC) filtration
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 45, Issue 9, September 2010, Pages 1543–1549
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us