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Scopolamine, anisodamine and hyoscyamine production by Brugmansia candida hairy root cultures in bioreactors

Paper ID Volume ID Publish Year Pages File Format Full-Text
34981 45064 2010 5 PDF Available
Title
Scopolamine, anisodamine and hyoscyamine production by Brugmansia candida hairy root cultures in bioreactors
Abstract

Hyoscyamine, anisodamine (6β-hydroxyhyoscyamine) and scopolamine are tropane alkaloids produced by plants belonging to the Solanaceae family such as Brugmansia candida. These alkaloids were traditionally used in medicine because of their anticholinergic activity. Further therapeutical properties for anisodamine were recently described renewing the interest in these alkaloids. The scaling-up of hairy root cultures is an interesting strategy for the pharmaceutical production of these compounds instead of the isolation from plants. In this work, B. candida hairy roots were cultured in a modified 1.5 L stirred tank in order to analyze an alternative production system of scopolamine and anisodamine. It was found that these cultures produced an increased biomass and alkaloids concentration compared to the processes carried out in Erlenmeyer flasks. Anisodamine was the predominant alkaloid reaching a maximum concentration of 10.05 ± 0.76 mg/g DW in modified bioreactor culture system. The results obtained in this work are potentially applicable for the rational scale-up of the process.

Keywords
HR, hairy root; DW, dry weight; FW, fresh weight; GI, growth index; Yp/x, alkaloid yield referred to biomass formed; Yp/s, alkaloid yield referred to substrate consumed; A, anisodamine; S, scopolamine; H, hyoscyamineHyoscyamine; Anisodamine; Scopolamine;
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Scopolamine, anisodamine and hyoscyamine production by Brugmansia candida hairy root cultures in bioreactors
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 45, Issue 9, September 2010, Pages 1577–1581
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us