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Antimetastatic effect of ganoderic acid T in vitro through inhibition of cancer cell invasion

Paper ID Volume ID Publish Year Pages File Format Full-Text
34990 45067 2010 7 PDF Available
Title
Antimetastatic effect of ganoderic acid T in vitro through inhibition of cancer cell invasion
Abstract

Ganoderma lucidum (GL) is known as a valuable herb in the search for anticancer lead compounds because it has been used for thousands of years as a traditional medication. Triterpenoids are the principal active components in this fungus. To examine the bioactivity of triterpene compounds, Ganoderic acid T (GA-T) was isolated from G. lucidum by high performance liquid chromatography (HPLC). In this study, we investigated the anti-invasion and antimetastasis mechanisms of GA-T in vitro. GA-T dose-dependently inhibited 95-D cells migration by wound healing assay. Results of cell aggregation and adhesion assay showed that GA-T could promote cell aggregation and simultaneously inhibit cell from adhering to extracellular matrix (ECM). In addition, GA-T suppressed MMP-2 and MMP-9 gene expression, hence, decreased protein level of MMP-2 and MMP-9 in 95-D cells, according to RT-PCR assay and Western blot assay. These results demonstrate that GA-T effectively inhibits tumor metastasis in vitro. According to the result of Western blot, the suppression of NF-κB activation likely abrogates the expression of MMP-2 and MMP-9, thus prevents tumor metastasis. These results suggest that GA-T has therapeutic potential against high metastatic lung carcinoma as a new agent.

Keywords
ECM, extracellular matrix; MMPs, matrix metalloproteinases; BS, bovine serum; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; EC50, half effective concentration; SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis; RT, r
First Page Preview
Antimetastatic effect of ganoderic acid T in vitro through inhibition of cancer cell invasion
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 45, Issue 8, August 2010, Pages 1261–1267
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering