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Optimization of single-column chromatographic separation of fructooligosaccharides

Paper ID Volume ID Publish Year Pages File Format Full-Text
34999 45067 2010 5 PDF Available
Title
Optimization of single-column chromatographic separation of fructooligosaccharides
Abstract

An experimental study aimed at the design and optimization of single-column separation of fructooligosaccharides from mono- and disaccharides was carried out. Amberlite™ CR1320Ca, a cation-exchange resin with a calcium ion as the functional group was packed in two laboratory columns of different dimensions. Optimization parameters were the superficial velocity and the column load. In a smaller column, the maximal yield of fructooligosaccharides of 86% and the selectivity of separation of 82% were achieved for the column load of 2.0% and superficial velocity of 5.0 × 10−5 m s−1. At the same process conditions in a larger column, the yield and selectivity increased to 90%.

Keywords
Fructooligosaccharides; Elution chromatography; Cation exchanger; Poly(styrene-divinyl benzene) resin; Optimization; Process design
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 45, Issue 8, August 2010, Pages 1325–1329
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us