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Synthesis of biodiesel in column fixed-bed bioreactor using the fermented solid produced by Burkholderia cepacia LTEB11

Paper ID Volume ID Publish Year Pages File Format Full-Text
35003 45067 2010 7 PDF Available
Title
Synthesis of biodiesel in column fixed-bed bioreactor using the fermented solid produced by Burkholderia cepacia LTEB11
Abstract

We produced a lipase from Burkholderia cepacia in solid-state fermentation and used it to catalyze the synthesis of biodiesel in a fixed-bed reactor. In the solid-state fermentation step, a 50:50 (by mass) mixture of sugarcane bagasse and sunflower seed meal gave 234 units of pNPP-hydrolyzing activity per gram of dry solids at 96 h. This fermented solid was lyophilized and delipidated, packed into a column and used to catalyze the synthesis of biodiesel through the ethanolysis of soybean oil in a medium free of co-solvents, with the reaction mixture being continuously circulated through the column. The best conversion was 95% after 46 h, which was obtained at 50 °C, with an alcohol:oil molar ratio of 3:1, alcohol addition in two steps and the addition of 1% of (m/m) water to the reaction medium. This system has potential to decrease the costs of enzyme-catalyzed transesterification reactions.

Keywords
Biodiesel; Fixed-bed reactor; Solid-state fermentation; Lipase; Burkholderia cepacia
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Synthesis of biodiesel in column fixed-bed bioreactor using the fermented solid produced by Burkholderia cepacia LTEB11
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 45, Issue 8, August 2010, Pages 1348–1354
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us