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The use of a new mobile phase, with no multivalent cation binding properties, to differentiate extracellular polymeric substances (EPS), by size exclusion chromatography (SEC), from biomass used for wastewater treatment

Paper ID Volume ID Publish Year Pages File Format Full-Text
35012 45067 2010 7 PDF Available
Title
The use of a new mobile phase, with no multivalent cation binding properties, to differentiate extracellular polymeric substances (EPS), by size exclusion chromatography (SEC), from biomass used for wastewater treatment
Abstract

The fingerprints of extracellular polymeric substances (EPS) extracted from different types of biomass used for wastewater treatment (i.e., activated sludge, filamentous activated sludge, anaerobic granular sludge, anaerobic flocculated sludge) were studied by size exclusion chromatography (SEC) with Amersham Biosciences Superdex 200 10/300 GL column with a theoretical resolving range of 10–600 kDa. A new mobile phase, which does not display binding properties for multivalent cations, was previously optimized. This mobile phase contained 75 mM Hepes buffer at pH 7 with 15% acetonitrile (v/v) and was selected to minimize ionic and hydrophobic interactions between the molecules that make up the EPS and the column packing.When EPS extracted from similar sludges is analyzed using different mobile phases, the number of chromatographic peaks obtained is quite similar, and differences are mainly observed in the relative absorbance of the chromatographic peaks. However, very different chromatograms (number and relative absorbance of chromatographic peaks) are obtained for EPS extracted from different types of sludges. Furthermore, when dysfunctions, such as filamentous bulking in the activated sludge, occur in a bioreactor, they also induce strong variations in chromatographic profiles.

Keywords
Wastewater processes; Extracellular polymeric substances; Size exclusion chromatography; Fingerprints
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The use of a new mobile phase, with no multivalent cation binding properties, to differentiate extracellular polymeric substances (EPS), by size exclusion chromatography (SEC), from biomass used for wastewater treatment
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 45, Issue 8, August 2010, Pages 1415–1421
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us