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In situ quantification of microcarrier animal cell cultures using near-infrared spectroscopy

Paper ID Volume ID Publish Year Pages File Format Full-Text
35014 45067 2010 5 PDF Available
Title
In situ quantification of microcarrier animal cell cultures using near-infrared spectroscopy
Abstract

In-line monitoring tools are still required to understand and control animal cell processes, particularly in the case of vaccine production. Here, in situ near-infrared spectroscopy (NIRS) quantification of components in culture media was performed using microcarrier-based cultivations of adherent Vero cells. Because microcarriers were found to interfere with NIRS spectra acquisition, a suitable and innovative in situ calibration was developed for bioreactor cultures. A reliable and accurate NIRS technique for the quantification of glucose and lactate was established, with a calibration standard error of 0.30 and 0.21 g l−1, respectively. The robustness of this method was evaluated by performing NIRS calibration with operating conditions similar to those of industrial processes, including parameters such as microcarrier concentrations, cell seeding states and changes in analyte concentration due to feed and harvest strategies. Based on this calibration procedure, the predicted analyte concentrations in unknown samples was measured by NIRS analyses with an accuracy of 0.36 g l−1 for glucose and 0.29 g l−1 for lactate.

Keywords
Adherent animal cell process; Vero cell; Near-infrared spectroscopy; Microcarriers; In situ monitoring
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In situ quantification of microcarrier animal cell cultures using near-infrared spectroscopy
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 45, Issue 8, August 2010, Pages 1427–1431
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us