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Characterization of a hymenoptaecin-like antimicrobial peptide in the parasitic wasp Nasonia vitripennis

Paper ID Volume ID Publish Year Pages File Format Full-Text
35017 45069 2010 8 PDF Available
Title
Characterization of a hymenoptaecin-like antimicrobial peptide in the parasitic wasp Nasonia vitripennis
Abstract

Hymenoptaecin is a Hymenoptera insect-specific, glycine-rich antimicrobial peptide (AMP) found in non-parasitic bees. Here, we describe a unique hymenoptaecin-like gene (named nahymenoptaecin-1) in the parasitic wasp Nasonia vitripennis, which codes for a larger protein precursor with a carboxyl-terminal hymenoptaecin-like domain (HLD) similar to the bee hymenoptaecin. We recombinantly produced its full-length bioactive form as well as 1–33 and 34–98 fragments (named HLD-n and HLD-c, respectively). Recombinant HLD exhibited activity against Gram-negative and Gram-positive bacteria at micromolar concentrations. Compared to the full-length peptide, HLD-c possessed similar potency in inhibiting the growth of Stenotrophomonus but had a narrower antibacterial spectrum, whereas HLD-n only displayed weak effect on Stenotrophomonus, suggesting that HLD-n is a crucial determinant for bacterial target selectivity while HLD-c represents its active unit for the whole molecule. Circular dichroism analysis combined with ab initio structure prediction by Robetta indicated that HLD-n adopts a random coil conformation whereas glycine-rich HLD-c forms a loose β-sheet structure. Relative to bee hymenoptaecin, the upstream region of HLD contains two accurately repeated proline-rich AMP-like peptides instead of an acidic propeptide. Such difference could be a consequence of exon shuffling of autonomous modules after speciation.

Keywords
AMP, antimicrobial peptide; CD, circular dichroism; EK, enterokinase; HLD, hymenoptaecin-like domain; IMD, immune deficiency; MALDI-TOF, matrix-assisted laser desorption/ionization tandem time-of-flight; MW, molecular weight; PC, proprotein convertase; PP
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Characterization of a hymenoptaecin-like antimicrobial peptide in the parasitic wasp Nasonia vitripennis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 45, Issue 2, February 2010, Pages 139–146
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us