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Fast and efficient purification of chloroperoxidase from C. fumago

Paper ID Volume ID Publish Year Pages File Format Full-Text
35035 45069 2010 5 PDF Available
Title
Fast and efficient purification of chloroperoxidase from C. fumago
Abstract

Chloroperoxidase (CPO) from Caldariomyces fumago is a highly versatile and synthetically important enzyme. Nevertheless, the actual technical application is limited, mainly due to the high costs for purified preparations. Here, a novel fast and very efficient method for the purification of CPO has been developed. It involves separation of CPO from black fungal pigment and most side proteins in aqueous biphasic systems followed by a single chromatographic step. A residual yield of ∼70.4% and a specific activity of 2900 U/mg were obtained. Sufficient purity for technical application was already achieved after biphasic extraction, where CPO recovery from culture broth yielded about 100%. Time required for purification ranges between 30 min and 1 day depending on the desired degree of purity. Thus, a protocol is presented that reduces consumption of time and material and enhances the enzyme quality.

Keywords
Biocatalysis; Enzyme; Purification; Aqueous biphasic system; Chloroperoxidase
First Page Preview
Fast and efficient purification of chloroperoxidase from C. fumago
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 45, Issue 2, February 2010, Pages 279–283
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering