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Biodegradation of toluene at high initial concentration in an organic–aqueous phase bioprocess with nitrate respiration

Paper ID Volume ID Publish Year Pages File Format Full-Text
35041 45070 2010 5 PDF Available
Title
Biodegradation of toluene at high initial concentration in an organic–aqueous phase bioprocess with nitrate respiration
Abstract

An aerobic organic–aqueous system with forced aeration was shown to be inefficient in preventing significant volatile aromatic compounds loss in gassed systems since air sparging in n-hexadecane under abiotic conditions could reduce the toluene concentration from 2.1 g/L to about 0.5 g/L in 3 days with a gassing rate of 1VVM at 20 °C. However, the presence of such an organic phase was found to significantly reduce substrate loss in aerobic conditions in comparison to pure aqueous systems. It was thus decided to develop a new bioprocess based on an anaerobic microbial system operated in an organic–aqueous phase with nitrate respiration. The denitrifying bacterium used, Thauera aromatica K172, was produced by cultivation on sodium benzoate as carbon source under anaerobic conditions. This cultivated biomass (1.5 g/L) was shown to retain its ability to efficiently metabolize toluene in a biphasic medium without any significant loss of organic compound in the gas phase. Toluene biodegradation was thus performed in a biphasic system using a fed-batch technique involving sequential adding of both toluene and nitrate. The reaction rate with an initial concentration of toluene close to 14.5 g/L in hexadecane was found to be close to 0.5 g/L day and the molar stoichiometry of solute metabolization to nitrate reduction was close to 1:6. This work demonstrated that the denitrifying bacteria could efficiently degrade toluene in hexadecane–aqueous phase systems in which toxic compound release in the environment was prevented.

Keywords
Monoaromatic hydrocarbon; Biphasic system; Partition coefficient; Air sparging; Anaerobic bioreactor; Thauera aromatica
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Biodegradation of toluene at high initial concentration in an organic–aqueous phase bioprocess with nitrate respiration
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 45, Issue 11, November 2010, Pages 1758–1762
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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