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Enhanced beauvericin production with in situ adsorption in mycelial liquid culture of Fusarium redolens Dzf2

Paper ID Volume ID Publish Year Pages File Format Full-Text
35055 45071 2009 5 PDF Available
Title
Enhanced beauvericin production with in situ adsorption in mycelial liquid culture of Fusarium redolens Dzf2
Abstract

Fusarium redolens Dzf2, an endophytic fungal species, is a high producer of the antibiotic compound beauvericin (BEA). However, the BEA produced by the F. redolens Dzf2 fungus was retained mainly as an intracellular product. This study was to evaluate an integrated fermentation-in situ product recovery process for enhancement of BEA production in F. redolens Dzf2 myelical culture. A macroporous polystyrene resin (X-5) was selected as the sorbent and added to the mycelial culture flasks (enclosed in a nylon bag). With 2 g resin added to 40 ml medium in each flask in the early stationary growth phase (day 5), the volumetric BEA yield (on day 7) was increased from 194 to 265 mg l−1, with 65% being adsorbed to the resin phase. With resin renewal plus glucose feeding (on day 7), the BEA production was increased even more dramatically to 400 mg l−1 (on day 9), double of the yield in the batch control culture. The results show that in situ adsorption was an effective strategy for enhancing the BEA production and also facilitating its recovery in the mycelial liquid culture.

Keywords
Beauvericin; Mycelial culture; Shake-flask; In situ adsorption; Polymeric resin; Fermentation process
First Page Preview
Enhanced beauvericin production with in situ adsorption in mycelial liquid culture of Fusarium redolens Dzf2
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 44, Issue 10, October 2009, Pages 1063–1067
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering