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Purification of lipase derived from Burkholderia pseudomallei with alcohol/salt-based aqueous two-phase systems

Paper ID Volume ID Publish Year Pages File Format Full-Text
35058 45071 2009 5 PDF Available
Title
Purification of lipase derived from Burkholderia pseudomallei with alcohol/salt-based aqueous two-phase systems
Abstract

Alcohol/salt-based aqueous two-phase systems (ATPSs) were used to recover lipase derived from Burkholderia pseudomallei (B. pseudomallei). Nine biphasic systems, comprised of an alcohol-based top phase (ethanol, 2-propanol and 1-propanol) and a salt-based bottom phase (ammonium sulfate, potassium phosphate and sodium citrate), were evaluated for their effectiveness in lipase recovery. The stability of lipase in each of the solutions was tested, and phase diagrams were constructed for each system. The optimum partition efficiency for the purification of lipase was obtained in an ATPS of 16% (w/w) 2-propanol and 16% (w/w) phosphate in the presence of 4.5% (w/v) NaCl. The purified lipase had a purification factor of 13.5 and a yield of 99%.

Keywords
Lipase; Aqueous two-phase system; Alcohol/salt system; Purification; Burkholderia pseudomallei
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Purification of lipase derived from Burkholderia pseudomallei with alcohol/salt-based aqueous two-phase systems
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 44, Issue 10, October 2009, Pages 1083–1087
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us