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Enzymatic synthesis of green notes with hydroperoxide-lyase from olive leaves and alcohol-dehydrogenase from yeast in liquid/gas reactor

Paper ID Volume ID Publish Year Pages File Format Full-Text
35063 45071 2009 6 PDF Available
Title
Enzymatic synthesis of green notes with hydroperoxide-lyase from olive leaves and alcohol-dehydrogenase from yeast in liquid/gas reactor
Abstract

Hydroperoxide-lyase is a suitable enzyme for the biocatalytic production of six-carbon aldehydes which are responsible for the “green notes” in many fruits and vegetables. The hydroperoxide-lyase isolated from olive leaves is used in this work. The effects of pH, temperature, and substrate and enzyme concentrations on hexenals generation were optimized. The main objective of this paper consists on the elaboration of a biocatalytic procedure for the synthesis of flavors with green odor characteristics. For this purpose an enzymatic liquid/gas reactor, where the synthesis of C6-compounds was coupled to their extraction, has been proposed. Hexenals were produced in two steps: (1) 13 hydroperoxy-linolenic acid was produced from hydrolyzed linseed oil in presence of soybean lipoxygenase. (2) 3Z- and 2E-hexenals (up to 0.36 g kg−1 of reaction medium) were produced from 13 hydroperoxy-linolenic acid in presence of olive hydroperoxide-lyase at 50% yield. The hexenals were successfully reduced into their corresponding alcohols by adding yeast cells Saccharomyces cerevisiae containing alcohol-dehydrogenase activity to the same reactor. Significant amounts of 3Z-hexenol (up to 3.54 g kg−1 of olive leaves) were produced and extracted at a yield of 47.7% and with high purity when permeabilized yeast cells were used.

Keywords
Alcohol-dehydrogenase; Biosynthesis; Green note; Hydroperoxide-lyase; Liquid/gas bioreactor; Volatile extraction
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Enzymatic synthesis of green notes with hydroperoxide-lyase from olive leaves and alcohol-dehydrogenase from yeast in liquid/gas reactor
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 44, Issue 10, October 2009, Pages 1122–1127
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us