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Production and characterization of a C15-surfactin-O-methyl ester by a lipopeptide producing strain Bacillus subtilis HSO121

Paper ID Volume ID Publish Year Pages File Format Full-Text
35067 45071 2009 8 PDF Available
Title
Production and characterization of a C15-surfactin-O-methyl ester by a lipopeptide producing strain Bacillus subtilis HSO121
Abstract

A surfactin mono-methyl ester was isolated from the cell broth of Bacillus subtilis HSO121 by acid precipitation, methanol extraction and two-step chromatographic methods. The structure was analyzed by GC/MS, HPLC, ESI Q-TOF MS/MS and NMR. It indicates that this ester is a C15-surfactin-O-methyl ester of which the fatty acid portions are composed of iso-C15 and anteiso-C15 β-hydroxy fatty acids. Based on the critical micelle concentration (CMC) in phosphate buffered saline (PBS, pH 8.0) and the IC50 value on Hela cell lines, the C15-surfactin-O-methyl ester has a stronger surface activity and lower antitumoral effect than C15-surfactin does. It is found that the Glu residues of surfactin-like lipopeptides play a role in their anti-proliferative effects on Hela cells. The possible antitumoral activity of surfactin is discussed in relation to their structures.

Keywords
Surfactin; Bacillus subtilis; Structure; Surface activity; Hela cell; Carboxyl group
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Production and characterization of a C15-surfactin-O-methyl ester by a lipopeptide producing strain Bacillus subtilis HSO121
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 44, Issue 10, October 2009, Pages 1144–1151
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
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