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Increase in lipids production by Pichia angusta DL-1 utilizing the chemostat under double limitation of heterologous nutrients

Paper ID Volume ID Publish Year Pages File Format Full-Text
3507 173 2012 5 PDF Available
Title
Increase in lipids production by Pichia angusta DL-1 utilizing the chemostat under double limitation of heterologous nutrients
Abstract

For many years there has been industrial interest to produce microbial oils through oleaginous microorganisms. Its fatty acids composition is similar to vegetable oils, so that the use of microbial polyunsaturated fatty acids has been very important in animal nutrition and is currently considered the potential feedstock for biodiesel, therefore any significant improvement in the process of lipid synthesis will reduce costs of production at industrial level. We investigated the use chemostat under different heterologous double nutrient limitations, that is, nutrients that meet different physiological requirements, in order to achieve a higher production lipid accumulation in oleaginous yeast. We used Pichia angusta DL-1 grown in a chemostat under carbon/phosphorus, carbon/magnesium, nitrogen/phosphorus and nitrogen/magnesium double limitations at five different dilution rates. The largest accumulation of lipids occurred under nitrogen/phosphorus limitation at a dilution rate of 0.03 h−1 and carbon/magnesium limitation at a dilution rate of 0.07 h−1. At that condition lipid content was twice the one obtained in batch culture and 15% larger than under simple limitation. Finally, under carbon/magnesium limitation the highest lipid productivity (0.011 g/L h) was obtained with a cell productivity of 0.033 g/L h.

► The double limitation of heterologous nutrients can increase the lipid content of the yeast Pichia angusta DL-1. ► The largest accumulation of lipids occurred under nitrogen/phosphorus limitation and carbon/magnesium limitation. ► The content of lipids was twice the one obtained in batch culture and 15% larger than under simple limitation. ► Carbon and magnesium double limitation as the most adequate condition to obtain the highest lipid and cell volumetric productivities. ► The double limited chemostat can be used as an important tool for significantly improve the industrial production of SCO.

Keywords
Pichia angusta; Chemostat; Lipid accumulation; Double nutrient limitations; Fatty acids; Biodiesel
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 67, 15 August 2012, Pages 83–87
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us