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Growth and lipid production of Umbelopsis isabellina on a solid substrate—Mechanistic modeling and validation

Paper ID Volume ID Publish Year Pages File Format Full-Text
35078 45073 2012 15 PDF Available
Title
Growth and lipid production of Umbelopsis isabellina on a solid substrate—Mechanistic modeling and validation
Abstract

Microbial lipids are an interesting feedstock for biodiesel. Their production from agricultural waste streams by fungi cultivated in solid-state fermentation may be attractive, but the yield of this process is still quite low. In this article, a mechanistic model is presented that describes growth, lipid production and lipid turnover in a culture of Umbelopsis isabellina on κ-carrageenan plates containing the monomers glucose and alanine as C-source and N-source, respectively, and improves the understanding of the complex solid-state system. The model includes reaction kinetics and diffusion of glucose, alanine and oxygen. It is validated empirically and describes the different phases of the culture very well: exponential growth, linear growth because of oxygen limitation, accumulation of lipids and carbohydrates after local N-depletion and turnover of lipids after local C-depletion. Extending the model with an unidentified extracellular product improved the fit of the model to the data. The model shows that oxygen limitation is extremely important in solid-state cultures using monomers, and explains the difference in production rate with submerged cultures. However, the results also show that the specific lipid production rate in solid-state cultures is much lower than in submerged cultures, which results in a low lipid yield.

► We modeled growth and lipid production in oleaginous fungi on a solid substrate. ► The model includes diffusion of nutrients and oxygen in the substrate and the fungal biofilm. ► The model was validated with carrageenan plate cultures of Umbelopsis isabellina. ► Oxygen is the rate-limiting nutrient in the system. ► The specific lipid production rate found was much lower than in submerged cultures.

Keywords
Umbelopsis isabellina; Oleaginous fungi; Mechanistic model; Lipid; Solid substrate
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Growth and lipid production of Umbelopsis isabellina on a solid substrate—Mechanistic modeling and validation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 47, Issue 8, August 2012, Pages 1228–1242
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us