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Towards a commercially potential process: Enzymatic recovery of phytosterols from plant oil deodoriser distillates mixture

Paper ID Volume ID Publish Year Pages File Format Full-Text
35081 45073 2012 7 PDF Available
Title
Towards a commercially potential process: Enzymatic recovery of phytosterols from plant oil deodoriser distillates mixture
Abstract

In order to examine the industrial potential to indirectly isolate phytosterols from deodoriser distillates (DODs), enzymatic transesterification of an industrial rapeseed and soybean oil DOD mixture with bioethanol was investigated using commercial lipases and a few newly immobilised preparations of lipases. The lipases from different sources and differing preparation forms were evaluated, in terms of thermostability, enzyme efficiency, and toleration of ethanol. Lipozyme 435 and Lipozyme NS-40044 TLL were found to be most effective biocatalysts in catalysing ethanolysis of glycerides and steryl esters from DODs. The optimum conditions are 10% enzyme load (wt% of DODs), ethanol/DODs of 3.0:1.0 (mol/mol), water content 0.125% (based on the weight of total mixture), and reaction at 30 °C for 5 h. The results demonstrated that >95% sterols can be recovered as free form (>85% sterol esters were liberated as free sterols within 4 h). With this process, the system was simplified as fatty acid ethyl esters and free sterol as major components, where free sterols can be recovered via solvent extraction or molecular distillation. Furthermore, a reuse study of enzyme in consecutive batch reactions demonstrated an excellent operation stability and reusability of Lipozyme 435 and Lipozyme NS-40044 TLL with the developed process. This work indicated that the industrially refined waste DODs can be directly subjected to an enzymatic process for high efficacy recovery of phytosterol without any pre-process, driven by robust lipase preparations.

► New lipase preparations examined for enzymatic ethanolysis of DODs. ► Lipozyme 435 and Lipozyme NS-40044 TLL are most effective biocatalysts. ► Reaction completed in 4 h at 30 °C with 10% (wt%) enzyme load. ► 95% phytosterols expected to be recovered as free form after enzymatic process.

Keywords
Enzymatic recovery; Phytosterols; Fatty acid ethyl ester; Deodoriser distillate (DOD); Lipase
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 47, Issue 8, August 2012, Pages 1256–1262
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us