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Purification and characterization of an extracellular carbonic anhydrase from Pseudomonas fragi

Paper ID Volume ID Publish Year Pages File Format Full-Text
35102 45074 2009 5 PDF Available
Title
Purification and characterization of an extracellular carbonic anhydrase from Pseudomonas fragi
Abstract

Extracellular carbonic anhydrase was purified from Pseudomonas fragi isolated from CaCO3 enriched soil samples. The enzyme is induced in presence of CaCO3 and is envisaged to play an important role in bicarbonate ion transport. The 75% ammonium sulphate dialysate was purified by single step affinity chromatography with 86% yield. It is a trimeric protein having a subunit molecular weight of 31.0 kDa and was stable at pH 7.0–8.5 and temperature 35–45 °C. Lead, mercury and EDTA had an inhibitory effect on CA activity, whereas zinc, iron and cadmium increased it. The presence of esterase activity along with IC50 of sulphonamides and anionic inhibitors indicated that CA from P. fragi belonged to α-class. The CA stability in presence of different salts, as well as in alkaline pH and high temperature makes it a potential candidate to be exploited for biomimetic CO2 sequestration.

Keywords
Carbonic anhydrase; Pseudomonas fragi; Purification; Characterization; Alkaliphilic; Affinity chromatography
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Purification and characterization of an extracellular carbonic anhydrase from Pseudomonas fragi
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 44, Issue 11, November 2009, Pages 1293–1297
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Price was $35.95
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