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A global positive regulator afsR2 stimulates tautomycetin production via pathway-specific regulatory gene over-expression in Streptomyces sp. CK4412

Paper ID Volume ID Publish Year Pages File Format Full-Text
35103 45074 2009 4 PDF Available
Title
A global positive regulator afsR2 stimulates tautomycetin production via pathway-specific regulatory gene over-expression in Streptomyces sp. CK4412
Abstract

Tautomycetin (TMC) is a T cell-specific immunosuppressant with a unique ester bond linkage between a terminal cyclic anhydride and a linear polyketide chain. Since an afsR2 was proved to be a global antibiotics-stimulating regulatory gene in various Streptomyces species, an afsR2-induced TMC productivity stimulation was investigated in a TMC-producing Streptomyces sp. CK4412 strain. An afsR2 gene was cloned under the influence of a strong constitutive ermE* promoter in an integrative expression vector, followed by its conjugation into the Streptomyces sp. CK4412. Comparing TMC productivity and antifungal activity of the wild type and the afsR2-containing ex-conjugant revealed that afsR2 over-expression stimulated TMC production approximately 2.4-fold in Streptomyces sp. CK4412. Based on both RT-PCR and real-time RT-PCR analyses, an afsR2 over-expression significantly stimulated the expression of a TMC-specific positive regulatory gene, tmcN. This implies that the stimulatory effect of afsR2 functions in Streptomyces sp. CK4412 via up-regulation of a TMC pathway-specific positive regulatory gene over-expression.

Keywords
Streptomyces; Positive regulator; afsR2; Productivity-improvement; Tautomycetin; Immunosuppressant
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A global positive regulator afsR2 stimulates tautomycetin production via pathway-specific regulatory gene over-expression in Streptomyces sp. CK4412
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 44, Issue 11, November 2009, Pages 1298–1301
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us