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Purification of a novel cysteine protease, procerain B, from Calotropis procera with distinct characteristics compared to procerain

Paper ID Volume ID Publish Year Pages File Format Full-Text
35119 45076 2010 8 PDF Available
Title
Purification of a novel cysteine protease, procerain B, from Calotropis procera with distinct characteristics compared to procerain
Abstract

Proteases have applications in food, detergent and pharmaceutical industries. A novel protease has been purified from the latex of Calotropis procera and characterized. As another cysteine protease, procerain, is reported from the same source, the newly purified enzyme was named as procerain B. The enzyme shows distinct properties compared to procerain, in terms of cleavage recognition site, immunological properties and other physical properties like molecular weight, isoelectric point, etc. The newly purified enzyme shows a broad optimum pH (6.5–8.5) as well as broad optimum temperature (40–60 °C). Additionally, the enzyme retains its activity where most of other proteases are not active. Moreover, the enzyme appeared to be very efficient in hydrolysis of blood stain and may have potential application in detergent industries. Simple and economic purification of procerain B, together with easy availability of latex, makes the large-scale production of procerain B possible, thus enables to explore various industrial as well as biotechnological applications.

Keywords
BAPA, NR-benzoylarginine p-nitroanilide; DFP, diisopropylfluorophosphate; DTNB, 5,5′-dithiobis(2-nitrobenzoic acid); DTT, dithiothreitol; EDTA, ethylenediaminetetraacetic acid; EGTA, glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid; ELISA, enzyme-
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Purification of a novel cysteine protease, procerain B, from Calotropis procera with distinct characteristics compared to procerain
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 45, Issue 3, March 2010, Pages 399–406
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us