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Direct binding glucoamylase onto carboxyl-functioned magnetic nanoparticles

Paper ID Volume ID Publish Year Pages File Format Full-Text
3512 173 2012 6 PDF Available
Title
Direct binding glucoamylase onto carboxyl-functioned magnetic nanoparticles
Abstract

A novel and efficient immobilization of glucoamylase from Aspergillus niger has been developed by using carboxyl functioned magnetic Fe3O4 nanoparticles (CMNPs) as support. The CMNPs were prepared by co-precipitation of Fe2+/Fe3+ with oleic acid as surfactant and consequent oxidation of CC into COOH by KMnO4 solution in situ. And then glucoamylase was directly bonded onto the magnetic nanoparticles. The bonding was verified by Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis. The immobilization process was investigated by examining immobilization time, enzyme concentration and pH. As a result, it could gain an enzyme loading with 120 mg glucoamylase/g magnetic nanoparticles and specific activity of 90 U/mg protein while 1 mg/mL glucoamylase and 10 mg/mL ferrofluid were mixed at pH 6.0 and 25 °C for 16 h. The immobilized enzyme presented a same or better storage, pH and thermal stability than the free enzyme and could be reused. And the residual activity was about 40% after 8 cycles. The Michaelis constant (Km) showed that affinity of immobilized enzyme for binding substrate was lower than free enzyme.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Carboxyl functioned magnetic Fe3O4 nanoparticles (CMNPs) as support. ► The effects of immobilization of condition on the activity of immobilized enzyme were investigated. ► Catalysis characteristics of immobilized glucoamylase were compared with free enzyme.

Keywords
Enzyme biocatalysis; Immobilized enzymes; Kinetic parameters; Magnetic nanoparticles; Glucoamylase
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 67, 15 August 2012, Pages 120–125
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us