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Efficient regioselective acylation of andrographolide catalyzed by immobilized Burkholderia cepacia lipase

Paper ID Volume ID Publish Year Pages File Format Full-Text
35121 45076 2010 4 PDF Available
Title
Efficient regioselective acylation of andrographolide catalyzed by immobilized Burkholderia cepacia lipase
Abstract

For the first time, PSL-C, an immobilized lipase from Burkholderia cepacia, was successfully applied to the regioselective acylation of andrographolide by vinyl acetate in acetone. FT-IR spectra demonstrated the occurrence of acylation reaction. The 13C NMR, ESI-MS and elemental analysis confirmed that the 14-acetylandrographolide was formed exclusively. Water activity and reaction temperature had a significant effect on the initial rate and the substrate conversion, but little effect on the regioselectivity of the reaction. The optimal water activity and reaction temperature were 0.11 and 50 °C, respectively. Under these conditions, the initial rate and substrate conversion were 50.2 mM h−1 and 99.0%, respectively, after a reaction time of around 4 h. Besides, immobilized lipase also displayed higher operational stability and 83.5% of its original activity was maintained after being reused for eight batches.

Keywords
Andrographolide; 14-Acetylandrographolide; Burkholderia cepacia lipase; Regioselective acylation; Organic media
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Efficient regioselective acylation of andrographolide catalyzed by immobilized Burkholderia cepacia lipase
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 45, Issue 3, March 2010, Pages 415–418
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us