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Religiosin C, a cucumisin-like serine protease from Ficus religiosa

Paper ID Volume ID Publish Year Pages File Format Full-Text
35144 45078 2012 8 PDF Available
Title
Religiosin C, a cucumisin-like serine protease from Ficus religiosa
Abstract

A serine protease was purified to homogeneity from the latex of Ficus religiosa. The enzyme, named religiosin C is a monomer with molecular mass of 80 kDa. The enzymatic activity of the protein was inhibited by serine protease inhibitors. Isoelectric point of the enzyme is pH 4.6 with optimum pH and temperature of pH 6–8 and 45–50 °C, respectively. The specific extinction coefficient (ε2801%) of the enzyme is 14.68 with 16 tryptophan, 20 tyrosine and 7 cysteine residues in its molecular structure. The enzyme shows broad substrate specificity and hydrolyzes both natural and synthetic substrates. The enzyme is highly stable over a broad range of pH and temperature as well as in the presence of high concentration of chemical denaturants, organic solvents and metal ions. The N-terminal residues of religiosin C exhibited considerable homology with cucumisin and other cucumisin/subtilisin-like serine proteases. The high milk-clotting ability of religiosin C supports its probable use in the food and other biotechnological industries.

► Religiosin C is novel milk-clotting serine protease from a vegetal source. ► Enzyme is active over a wide range of pH and temperature. ► Enzyme is highly stable against denaturants, organic solvents and metal ions. ► Enzyme exhibits high milk-clotting activity in alkaline medium. ► Enzyme is quite resistant to autodigestion at higher concentration.

Keywords
BSA, bovine serum albumin; CBB, coomassie brilliant blue; DEAE, diethylaminoethyl; DMSO, dimethyl sulfoxide; DTNB, 5,5-dithiobis (2-nitrobenzoicacid); DTT, di-thiothreitol; EDTA, ethylenediaminetetraacetic acid; EGTA, ethylene glycol-bis (raminoethyl ethe
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Religiosin C, a cucumisin-like serine protease from Ficus religiosa
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 47, Issue 6, June 2012, Pages 914–921
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us