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Biodegradation of di-n-butyl phthalate and expression of the 3,4-phthalate dioxygenase gene in Arthrobacter sp. ZH2 strain

Paper ID Volume ID Publish Year Pages File Format Full-Text
35147 45078 2012 5 PDF Available
Title
Biodegradation of di-n-butyl phthalate and expression of the 3,4-phthalate dioxygenase gene in Arthrobacter sp. ZH2 strain
Abstract

The Arthrobacter sp. ZH2 strain was found to efficiently degrade di-n-butyl phthalate, the optimal temperature and pH conversion conditions of which were 30 °C and 9.0, respectively. The degradation of DBP was best fitted by first-order kinetic equation and the half-life was 10.19 h. ZH2 preferentially utilized DBP rather than dimethyl phthalate (DMP) when a minimal salt medium was added with a mixture of phthalate acid esters (PAEs) and the degradation of DMP and DBP could not induce the degradation of di-n-octyl phthalate (DOP). Partial sequences of the 3,4-phthalate dioxygenase gene were amplified from ZH2. The expression ratio of 3,4-phthalate dioxygenase gene increased from 1-fold to 28.84-fold when the DBP concentration was increased from 0 to 500 mg per l. To our knowledge, this is the first report regarding the detection of the response of the 3,4-phthalate dioxygenase gene to different concentrations of DBP using reverse transcription quantitative PCR (RT–qPCR).

► Bacterial strain Arthrobacter sp. ZH2 was used to degrade DBP. ► The optimal pH for Arthrobacter sp. ZH2 to degrade DBP is 9.0. ► Partial 3,4-phthalate dioxygenase gene was successfully amplified. ► The expression of 3,4-phthalate dioxygenase gene was positively related to DBP concentration.

Keywords
Biodegradation; Di-n-butyl phthalate; 3,4-Phthalate dioxygenase gene; RTq PCR
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Biodegradation of di-n-butyl phthalate and expression of the 3,4-phthalate dioxygenase gene in Arthrobacter sp. ZH2 strain
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 47, Issue 6, June 2012, Pages 936–940
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us