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A “yellow” laccase with “blue” spectroscopic features, from Sclerotinia sclerotiorum

Paper ID Volume ID Publish Year Pages File Format Full-Text
35152 45078 2012 8 PDF Available
Title
A “yellow” laccase with “blue” spectroscopic features, from Sclerotinia sclerotiorum
Abstract

Reported here are the production, purification and characterization of a laccase from the phytophathogenic fungus Sclerotinia sclerotiorum. This laccase is identified by mass spectrometry with a sequence coverage of 74.9% (458/577 AA) revealing that the protein is identical or highly homologous to a predicted oxidoreductase from this species (A7EM18 in the Uniprot database); the closest homologous protein previously isolated from a fungus is the Melanocarpus albomyces, with only 35% identity. The UV–vis spectral features of this laccase classify it as a “yellow” one. The EPR spectrum nevertheless demonstrates resemblance to blue laccases – including the type 1 center not detectable in UV–vis spectra. The presence of type 3 coppers was proven by fluorescence spectrum and by 330 nm band in UV–vis. The purified laccase has an apparent molecular mass of 70 kDa and appears as a monomer. The values of KM and kcat were determined for ABTS, 2,6-dimethoxyphenol, p-phenylenediamine and guaicol and are typical of a laccase. The optimal pH value is around 4 except for ABTS, for which activity is linearly increasing with acidity. The high laccase activity in liquid culture makes S. sclerotiorum a useful source of laccase for practical applications.

► A new laccase was isolated from Sclerotinia sclerotiorum. ► This is the first to be classified as ‘yellow’ based on UV–vis yet ‘blue’ based on EPR. ► This laccase has a sequence not previously annotated as such in S. sclerotiorum. ► Good catalytic parameters and high activity in liquid culture offer promise for applications.

Keywords
Sclerotinia sclerotiorum; Yellow laccase; EPR spectroscopy; UV–vis spectroscopy
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A “yellow” laccase with “blue” spectroscopic features, from Sclerotinia sclerotiorum
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 47, Issue 6, June 2012, Pages 968–975
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us