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Combination use of ultrasound irradiation and ionic liquid in enzymatic isomerization of glucose to fructose

Paper ID Volume ID Publish Year Pages File Format Full-Text
35153 45078 2012 7 PDF Available
Title
Combination use of ultrasound irradiation and ionic liquid in enzymatic isomerization of glucose to fructose
Abstract

The synthetic effect of the combination use of ultrasound irradiation (UI) and ionic liquid (IL) on improving enzyme activity was studied when they were employed in isomerization of glucose to fructose by immobilized glucose isomerase (IGI, produced from streptomyces murinus and immobilized on silica). Both of UI and IL [EMIM][Cl], which was screened as the best medium for this reaction, were found to increase the enzyme activity in isomerization reaction. And a further increase of enzyme activity was observed by combination use of UI and IL. A systematic screening and optimization of the reaction parameters in ILs under UI on the IGI activity were performed. Under the optimum reaction conditions, 45.3% yield of fructose was achieved in 10 h under UI in [EMIM][Cl], compared to only 41.5% yield under stirring in [EMIM][Cl], 44.2% under UI without [EMIM][Cl] and 38.9% under stirring without [EMIM][Cl] in 12 h, respectively. High thermal stability and reusability of IGI was also observed under UI in [EMIM][Cl]. These results indicated that the combination use of UI and IL might be a fast and efficient method for enzymatic isomerization of glucose to fructose.

► Combination use of ultrasound irradiation and ionic liquid in enzymatic isomerization reaction. ► A synergistic effect of ultrasound irradiation and ionic liquid on improving enzyme activity. ► Increased thermal stability and reusability of isomerase.

Keywords
BMIM, 1-butyl-3-methylimidazolium; EMIM, 1-ethyl-3-methylimidazolium; OAc, acetic acid; DCA, dicyanamide; BF4, tetrafluoroborate; PF6, hexafluoroantimonate; OTf, trifluoromethanesulfonate; NTf2, bis{(trifluoromethyl)sulfony}amid; Cl, chloride; Br, bromide
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Combination use of ultrasound irradiation and ionic liquid in enzymatic isomerization of glucose to fructose
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 47, Issue 6, June 2012, Pages 976–982
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Don't Miss Today's Special Offer
Price was $35.95
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Price after discount Only $4.95
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