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Production of 6-pentyl-α-pyrone with Trichoderma atroviride and its mutant in a novel extractive liquid-surface immobilization (Ext-LSI) system

Paper ID Volume ID Publish Year Pages File Format Full-Text
35163 45079 2009 6 PDF Available
Title
Production of 6-pentyl-α-pyrone with Trichoderma atroviride and its mutant in a novel extractive liquid-surface immobilization (Ext-LSI) system
Abstract

A novel extractive fermentation procedure, tentatively named an extractive liquid-surface immobilization (Ext-LSI) system, for the production of water-insoluble secondary metabolites with fungi was developed. The system using a unique polymeric micro-material, a ballooned polyacrylonitrile microshpere (MS), was applied to the production of 6-pentyl-α-pyrone (6PP), a fungicidal secondary metabolite, with a newly isolated Trichoderma atroviride AG2755-5 and its nitrosoguanidine (NTG)-mutant. The resulted mutant AG2755-5NM398 was immobilized on the surface of a liquid medium by using the MS. Following the formation of a fungus-MS mat, low toxic hydrophobic solvent, dimethyl silicone oil, was added onto the fungus-MS mat. The optimum carbon and nitrogen sources were fructose and malt extract, respectively. The higher the initial medium pH, the more the 6PP-accumulation was observed. The best MS and extractive organic solvent were MFL-80SDE (non-coated type) and KF-96L-1CS (dimethyl silicone oil). Thus, produced 6PP was spontaneously extracted from cells to the organic phase to reach 7.1 g L−1 of the accumulation in the organic phase.

Keywords
Ext-LSI, extractive liquid-surface immobilization; MS, microsphere; 6PP, 6-pentyl-α-pyrone; NTG, nitrosoguanidine; LSI, liquid-surface immobilization; L–L IBR, liquid–liquid interface bioreactor; S–L IBR, solid–liquid interface bioreactorLiquid-surface cu
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Production of 6-pentyl-α-pyrone with Trichoderma atroviride and its mutant in a novel extractive liquid-surface immobilization (Ext-LSI) system
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 44, Issue 6, June 2009, Pages 625–630
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us