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An efficient system for pre-delignification of gramineous biofuel feedstock in vitro: Application of a laccase from Pycnoporus sanguineus H275

Paper ID Volume ID Publish Year Pages File Format Full-Text
35189 45080 2010 7 PDF Available
Title
An efficient system for pre-delignification of gramineous biofuel feedstock in vitro: Application of a laccase from Pycnoporus sanguineus H275
Abstract

An efficient system is presented for pre-delignifying cereal straw in vitro using laccase produced by Pycnoporus sanguineus H275. Under our experimental conditions, over 97% of the klason lignin was removed from 1 g wheat straw lignocellulose, without loss of Somogyi carbohydrate (reducing sugar). Microscopic observation of birefringence changes further indicated that the structure of the cellulose building blocks (microfibrils) of wheat straw scattered after pre-degradation, compared with the tightly arranged microfibrils in untreated control straw. The P. sanguineus H275-laccase-encoding gene was then cloned and successfully expressed in Pichia pastoris X-33, with a production of 3000 U/L after 6 days of cultivation. The identity of the expressed laccase was further confirmed by using MALDI-TOF-MS/MS. Homology modeling revealed that the native H275-laccase, predicted with five possible N-glycosylation sites, has extra cupredoxine domains and three common Cu-oxidase domains. Both kinetic characterization on the substrate ABTS and pre-delignification investigation on wheat straw lignocellulose showed that the activity of the recombinant laccase was nearly the same as that of the native laccase.

Keywords
Laccase; Lignin; Lignocellulose; White-rot; Pre-delignification; Pycnoporus sanguineus
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An efficient system for pre-delignification of gramineous biofuel feedstock in vitro: Application of a laccase from Pycnoporus sanguineus H275
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 45, Issue 7, July 2010, Pages 1141–1147
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us