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Efficient screening a high glutathione-content mutant of Saccharomyces cerevisiae by flow cytometry

Paper ID Volume ID Publish Year Pages File Format Full-Text
35193 45080 2010 4 PDF Available
Title
Efficient screening a high glutathione-content mutant of Saccharomyces cerevisiae by flow cytometry
Abstract

Screening for the high glutathione-content microorganisms is an important technique for industrial production of glutathione. In this study, the intracellular glutathione-content was investigated to correlate better with the fluorescence emission at around 490 nm in the yeast. Therefore, we developed an efficient flow cytometry method to screen for the higher glutathione-content mutant of Saccharomyces cerevisiae by measuring the intensity of fluorescence emitted from the cell. The library of mutants was generated by ethyl methanesulfonate mutagenesis and mutant G-143 was isolated which produced 35% higher glutathione than the parent strain in the flask culture, and the glutathione yield was 37% increased when G-143 was scaled up to a 5 L fermentor for glutathione was the intracellular product.

Keywords
Glutathione; Screening; Saccharomyces cerevisiae; Flow cytometry; Fermentation; Mutagenesis
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Efficient screening a high glutathione-content mutant of Saccharomyces cerevisiae by flow cytometry
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 45, Issue 7, July 2010, Pages 1168–1171
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us