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Characterization of protein hydrolysates and lipids obtained from black scabbardfish (Aphanopus carbo) by-products and antioxidative activity of the hydrolysates produced

Paper ID Volume ID Publish Year Pages File Format Full-Text
35204 45081 2010 7 PDF Available
Title
Characterization of protein hydrolysates and lipids obtained from black scabbardfish (Aphanopus carbo) by-products and antioxidative activity of the hydrolysates produced
Abstract

The preparation of protein hydrolysates from black scabbardfish (Aphanopus carbo) by-products (BSB) was studied as an alternative for the upgrading of this raw material. Different levels of Protamex™ were used in the hydrolysate preparation and the peptides obtained and the lipids present in each of the four fractions obtained after centrifugation of the hydrolysed material were characterized. The nitrogen solubilization achieved was around 83% and degree of hydrolysis (DH) was about 57%. A high proportion of the peptides had molecular weights lower than 1000 Da. The highest percentage of oil remained in the emulsion (50–65%) and the highest percentage of free oil obtained was ca. 36%. The fatty acid profile of lipids present in the BSB and in the different fractions was similar. The triacylglycerols were dominant in all fractions and the highest percentage of phospholipids was detected in the oil from sludge and hydrolysate, followed by the emulsion but they were not present in the free oil. Cholesterol and cholesterol esters were detected in all fractions at low levels. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity and the reducing power of BSB hydrolysates increased with increasing DH. However, the hydroxyl scavenging activity decreased with DH.

Keywords
Black scabbardfish; By-products; Fish protein hydrolysates; Lipids; Fatty acids; Antioxidative activity
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Characterization of protein hydrolysates and lipids obtained from black scabbardfish (Aphanopus carbo) by-products and antioxidative activity of the hydrolysates produced
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 45, Issue 1, January 2010, Pages 18–24
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us