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Modulation of protease activity to enhance the recovery of recombinant nucleocapsid protein of Nipah virus

Paper ID Volume ID Publish Year Pages File Format Full-Text
35221 45081 2010 5 PDF Available
Title
Modulation of protease activity to enhance the recovery of recombinant nucleocapsid protein of Nipah virus
Abstract

The nucleocapsid (N) protein of Nipah virus (NiV) expressed in Escherichia coli (E. coli) is antigenic and immunogenic. A method to enhance the recovery of recombinant N protein of NiV produced in E. coli is described. A bioinformatics tool, PeptideCutter was used to identify potential protease and cleavage sites from the amino acid sequences deduced from the published DNA sequence of the N protein of NiV. The size of degraded protein was estimated by using the Western blot and PeptideCutter analyse. The identified proteases were serine proteases, hence, a range of serine protease inhibitors were tested to improve the recovery of the N protein. The relative amount of N protein of NiV was 2-fold higher with the addition of PMSF, compared to the control sample (without any protease inhibitor supplementation).

Keywords
Endogenous protease; Protease inhibitor; Nucleocapsid protein; Nipah virus; Escherichia coli
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Modulation of protease activity to enhance the recovery of recombinant nucleocapsid protein of Nipah virus
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 45, Issue 1, January 2010, Pages 133–137
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us