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Arthrobacter sp. lipase immobilization on magnetic sol–gel composite supports for enantioselectivity improvement

Paper ID Volume ID Publish Year Pages File Format Full-Text
35227 45082 2009 7 PDF Available
Title
Arthrobacter sp. lipase immobilization on magnetic sol–gel composite supports for enantioselectivity improvement
Abstract

A bacterial lipase from Arthrobacter sp. (ABL: IIIM Jammu, India strain, MTCC No. 5125) has been immobilized on non-magnetic (Type A) and magnetic (Type B) supports derived from copolymerization of 3-aminopropyltriethoxysilane and tetraethylorthosilicate. Immobilized ABL presented 21–34 mg/g protein binding providing 30–75 units/g activity in Type A non-magnetic composites and 24–45 mg/g protein binding providing 35–90 units/g activity with Type B supports containing magnetic particles. Immobilized ABL preparations have shown enhanced stability at pH 5–9 and temperature up to 70 °C whereas free ABL is unstable under these conditions. Improved hydrolytic conversion as well as enantioselectivity were observed with acyl fluoxetine intermediate (ethyl 3-hydroxy-3-phenylpropanoate alkyl acylates) and chiral auxiliaryacyl 1-phenyl ethanol using immobilized ABL derivatives (ee ∼99%; 3–4-fold increase in E-values) as compared to ABL enzyme/cells (ee 93–98%). Introduction of magnetic particles in these supports has led to easier separation process with high product recovery yields.

Keywords
Arthrobacter sp. lipase; Immobilization; Sol–gel; Magnetic composite; Resolution; Enantioselectivity
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Arthrobacter sp. lipase immobilization on magnetic sol–gel composite supports for enantioselectivity improvement
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 44, Issue 2, February 2009, Pages 154–160
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us