fulltext.study @t Gmail

A novel transglycosylating β-galactosidase from Enterobacter cloacae B5

Paper ID Volume ID Publish Year Pages File Format Full-Text
35238 45082 2009 5 PDF Available
Title
A novel transglycosylating β-galactosidase from Enterobacter cloacae B5
Abstract

A strain of Enterobacter cloacae B5 producing β-galactosidase with transglycosylation activity was isolated from the soil. Its freeze-thawed cells synthesized galacto-oligosaccharides with a high yield of 55% from 275 g/L lactose at 50 °C for 12 h. A novel β-galactosidase capable of glycosyl transfer was purified from this strain. It was a homotetramer with molecular mass of about 442 kDa. The optimal pH and temperature for hydrolysis activity on o-nitrophenyl-β-d-galactopyranoside (oNPGal) were 6.5–10.5 and 35 °C, respectively. The enzyme showed a wide range of acceptor specificity for transglycosylation and catalyzed glycosyl transfer from oNPGal to various chemicals such as galactose, glucose, fructose, arabinose, mannose, sorbose, rhamnose, xylose, cellobiose, sucrose, trehalose, melibiose, inositol, mannitol, sorbitol and salicin, resulting in novel saccharide yields ranging from 0.8% to 23.5%. A gene encoding the enzyme was cloned and the recombinant enzyme from Escherichia coli had similar transglycosylation activity to the natural enzyme.

Keywords
Enterobacter cloacae B5; β-Galactosidase; Transglycosylation; Galacto-oligosaccharides; Galactose-containing chemicals
First Page Preview
A novel transglycosylating β-galactosidase from Enterobacter cloacae B5
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 44, Issue 2, February 2009, Pages 232–236
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us