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A response surface methodological study on prediction of glucosylation yields of thiamin using immobilized β-glucosidase

Paper ID Volume ID Publish Year Pages File Format Full-Text
35242 45082 2009 5 PDF Available
Title
A response surface methodological study on prediction of glucosylation yields of thiamin using immobilized β-glucosidase
Abstract

Response surface methodology was used to predict the glucosylation yields of thiamin using immobilized β-glucosidase. A Central Composite Rotatable Design (CCRD) of 32 experiments with immobilized β-glucosidase, thiamin, incubation period, buffer concentration and pH, as five independent variables was employed at five levels. A second-order polynomial equation was developed, the regression coefficient values of which exhibited a R2 value of 0.74. Contour plots explained the glucosylation behaviour of the enzyme through a reversal in glucosylation at a cross-over point corresponding to 60% (w/w d-glucose) immobilized β-glucosidase and 0.12 mM buffer concentration at pH 6. The highest conversion yield of 58% obtained experimentally compared well with the predicted yield of 52% under optimum conditions of 40% immobilized β-glucosidase, 0.55 mmol thiamin, 96 h incubation period and 0.16 mM buffer concentration at pH 7. Validation experiments carried out at certain random predictive conditions also showed good correspondence between experimental and predictive yields.

Keywords
CCRD; Glucosylation; Immobilized β-glucosidase; RSM; Reversal in glucosylation; Thiamin glucoside
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A response surface methodological study on prediction of glucosylation yields of thiamin using immobilized β-glucosidase
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 44, Issue 2, February 2009, Pages 251–255
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us