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Synthesis of prolyl-hydroxyproline using prolyl aminopeptidase from Streptomyces aureofaciens TH-3

Paper ID Volume ID Publish Year Pages File Format Full-Text
35249 45083 2011 5 PDF Available
Title
Synthesis of prolyl-hydroxyproline using prolyl aminopeptidase from Streptomyces aureofaciens TH-3
Abstract

Despite attention devoted to prolyl-hydroxyproline (Pro-Hyp) in nutraceuticals and pharmaceuticals, its enzymatic synthesis has not been achieved. This study investigated aminolysis reaction from proline esters or amide and hydroxyproline using prolyl aminopeptidases from Streptomyces aureofaciens TH-3 (PAP TH-3). The effects of pH, buffer concentration, reaction temperature, and the type and concentration of the acyl donor were examined for the aminolysis activity of PAP TH-3. The Pro-Hyp synthesis was conducted in alkaline conditions at low temperature. The type of acyl donor also affected the yield. Finally, optimum conditions were established as 5 μl of 1 M proline amide, 50 μl of 2 M hydroxyl-proline, 40 μl of 1200 mM boric buffer (pH 11), 5 μl of water containing 10 μg PAP TH-3, 4 °C and 3 h. Based on the acyl donor, 30% of the maximum yield was obtained.

Keywords
Prolyl-hydroxyproline; Pro-Hyp; Prolyl aminopeptidase; Aminolysis
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Synthesis of prolyl-hydroxyproline using prolyl aminopeptidase from Streptomyces aureofaciens TH-3
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 46, Issue 8, August 2011, Pages 1560–1564
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us