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Production of xylanase by Promicromonospora sp MARS with rice straw under non sterile conditions

Paper ID Volume ID Publish Year Pages File Format Full-Text
35256 45083 2011 5 PDF Available
Title
Production of xylanase by Promicromonospora sp MARS with rice straw under non sterile conditions
Abstract

Quantitative differentiation of the inoculated Promicromonospora sp MARS and indigenous microbial flora of rice straw is described using ERIC-PCR during the production of xylanase in non sterile solid state fermentation (NS-SSF) to reduce the cost of enzyme production. Under non-sterile solid state fermentation with rice straw, Promicromonospora sp MARS produced 85.0 IU/g of xylanase. DNA fingerprints of unknown bacterial isolates obtained on BHA + xylan exactly matched with that of Promicromonospora sp MARS. Using PCR based enumeration; population of Promicromonospora sp MARS was 67.5 × 105 cfu/g after 48 h which increased to 23 × 106 cfu/g after 96 h which coincided with the maximum xylanase activity as compared to 14 × 104 cfu/g in control after 96 h. Under submerged fermentation (SmF), after 48 h, Promicromonospora sp MARS produced maximum xylanase (42.2 IU/ml) using rice straw as substrate followed by 19.9 and 20.4 IU/ml when Promicromonospora sp MARS was grown on rice husk and wheat straw, respectively. Optimum pH and temperature of the crude xylanase were 8.0 and 65 °C. Crude enzyme was 95% stable for 180 min of incubation at pH 8.0 and was stable at 65 °C for 240 min.

Keywords
Non sterile solid state fermentation; ERIC-PCR; Xylanase and Promicromonospora sp MARS
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 46, Issue 8, August 2011, Pages 1614–1618
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us