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The limited deglucosylation process of β-glucosidase in Bacillus cereus H62L for biotransforming secoisolariciresinol diglucoside into mammalian lignans

Paper ID Volume ID Publish Year Pages File Format Full-Text
35259 45083 2011 9 PDF Available
Title
The limited deglucosylation process of β-glucosidase in Bacillus cereus H62L for biotransforming secoisolariciresinol diglucoside into mammalian lignans
Abstract

The amount of major lignans present in the alkaline extract of the defatted flaxseed (in mg/g) is as follows: secoisolariciresinol diglucoside (SDG, 109.80), p-coumaric acid glucoside (CAG, 70.21), ferulic acid glucoside (FAG, 68.81), secoisolariciresinol (SECO, 0.49), coumaric acid (CA, 0.37), and ferulic acid (FA, 0.85). When biotransformed, SDG yields two valuable mammalian lignans (MLG): enterodiol (END) and enterolactone (ENL). Bacillus cereus strain H62-L1 was isolated and tested for the process of bioconversion. Kinetic analysis revealed the sequential reaction SDG (A) → CAG (B) → END (C) → ENL (D) to be reversible at the first step with the generalized reaction type A⇄k2k1B⟶k3C⟶k4D.The obtained rate coefficients were as follows: k1 = 0.001–0.028 h−1, k2 = 0.006–0.077 h−1, k3 = 0.013 h−1, and k4 = 0.002 h−1, and the overall kinetic parameter k1/(k2 + k3) varied from 0.05 to 1.32, mostly not favoring the forward deglucosylation reaction. SDG with higher purity favored the forward reaction. In conclusion, to facilitate the production rate, we propose (i) using substrate SDG with high purity at a higher concentration; (ii) using an allosteric inhibitor to block the reverse reaction from SECO to SDG; or (iii) genetically modifying Bacillus cereus H62-L1.

Keywords
Flaxseeds; Secoisolariciresinol diglucoside; Enterodiol; Mammalian lignans; Glucosidase
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The limited deglucosylation process of β-glucosidase in Bacillus cereus H62L for biotransforming secoisolariciresinol diglucoside into mammalian lignans
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 46, Issue 8, August 2011, Pages 1632–1640
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us